Determination of Lactose by an Enzymatic Method
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چکیده
منابع مشابه
Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase.
We describe a highly sensitive and specific method for determining L-carnitine in serum by use of carnitine dehydrogenase (EC 1.1.1.108). The method involves a new enzymatic cycling technique with NADH, thio-NAD+, and carnitine dehydrogenase, and measures the increase of absorbance at 415 nm of thio-NADH produced at 37 degrees C during the reaction: [formula: see text] The calibration curve for...
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Facultad de Farmacia, Area de Nutrición y Bromatologı́a, Universidad de Santiago, 15706 Santiago de Compostela (Galicia), Spain; Facultad de Ciencias, Area de Quı́mica Analı́tica, Universidad de La Coruña, Campus de Zapateira s/n, 15071 La Coruña (Galicia), Spain; and Facultad de Ciencia y Tecnologı́a de los Alimentos y Ciencias Quı́micas, Area de Nutrición y Bromatologı́a, Universidad de Burgos, Pla...
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* Present address, Department of Biochemistry, University of California, Berkeley 4, California. 1 The following abbreviations have been used; THF, tetrahydrofolic acid; lo-formyl-THF, N*o-formyltetrahydrofolic acid; 5,10-methenyl-THF, the cyclic N6-NrOimidazolinium derivative of 5-formyl-THF (anhydroleucovorin or anhydrocitrovorum factor) ; ATP, adenosine triphosphate; ADP, adenosine diphospha...
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ENZYMATIC REACTIONS, BECAUSE of their great specificity, are among the most valuable analytic tools at the disposal of the chemist. The method for glucose here described represents an adaptation of such biologic reagents and is a distinct departure from sugar methods now in common use. It is based upon a unique principle communicated to us by Keston (1) prior to publication (2). Traditional met...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1985
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(85)81167-x